Plasmid
Part:BBa_K4193019
Designed by: Rui Shi Group: iGEM22_OUC-China (2022-09-29)
pET28a-GABA1-L1
used to detect if the riboswitch has desired functions
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1062
Illegal XbaI site found at 1205
Illegal PstI site found at 299 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1062
Illegal NheI site found at 1101
Illegal PstI site found at 299
Illegal NotI site found at 165 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1062
Illegal BglII site found at 1271
Illegal BamHI site found at 192
Illegal BamHI site found at 336
Illegal BamHI site found at 1068
Illegal XhoI site found at 158 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1062
Illegal XbaI site found at 1205
Illegal PstI site found at 299 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1062
Illegal XbaI site found at 1205
Illegal PstI site found at 299
Illegal NgoMIV site found at 1303
Illegal NgoMIV site found at 2891
Illegal NgoMIV site found at 3051
Illegal NgoMIV site found at 6098 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 3971
[edit]
Categories
Parameters
None |